Coding

Part:BBa_K1799009:Design

Designed by: Michael Flanagan   Group: iGEM15_Genspace   (2015-08-07)


LsrD (modularization optimized)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 90
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This version of the open reading frame has been optimized for modularization via silent mutations in segments of code sharing more than 83% homology with the Shine-Dalgarno sequence. It is otherwise identical to the wild type LsrD gene (https://parts.igem.org/Part:BBa_K1799005).


Source

This part was synthesized, and is based on E. coli genomic data retrieved via EcoGene: http://www.ecogene.org/gene/EG13808

References